What is a Fluorescence Microscope?

August 29, 2022by Kalstein

The microscope is an optical equipment used in the laboratories, it has the ability to observe at scales of approach, fulfilling the analysis of particles and the image obtained, is really an investigation on the composition of objects.

With respect to the fluorescence microscope, it allows light to radiate the sample, and then deflect the weaker light emitted from the image. It is a conventional optical equipment, has a fluorescence adapter, which allows performing the optical observation of the sample, both conventional and contrast with fluorescence.

The captured image is the result of the electromagnetic emission expressed by the molecules that have attracted the primary excitation, and remitted a light with greater wavelength. To pass only the desired secondary emission, appropriate filters must be placed under the capacitor and above the lens.

Principles of the Fluorescence Microscope

The fluorescence microscope operates on the luminosity principle of fluorescence. This research technique is distinguished by the high level of amplitude that can be achieved with its analysis technique. In fluorescence, two chemical elements interact. These are:

  • Fluorochrome: It is a core that basically functions as a dye, which creates a certain contrast in the area of interest of study or observation.
  • Fluorophore: A protein that contains fluorescence characteristics and behaves like a type of reflector. Since the same, it attracts energy with short amplitude waves and repeats them with a higher amplitude of waves than received.

In this sense, it has a lighting method supplied by a halogen light emitter, which passes through a filter that normalizes the waves with amplitude necessary to stimulate fluorochrome. The result is reflected in a dichroic and affects the sample.

Stimulation of fluorochrome repeats energy at a wavelength greater than received. This light energy transmits you through the dichroic. Finally, it goes through a second filter that selects only the waves with amplitude emitted by fluorochrome.

Functions of the Fluorescence Microscope

Firstly, the microscope has a filter that allows the entry of radiation with a specific wavelength, which is the same as that of the fluorescent material. Then, the radiation impacts the atoms in the specimen, and the electrons are excited at a higher energy level. When relaxed to a lower level, they emit light, which allows to be detectable, and the fluorescence emitted from the sample is separated from the brighter excitation with a second filter and is met, thanks to the emission of the lesser light, with a wavelength greater than that used for illumination.

It also allows us to determine the distribution of a single species of molecule, its quantity and location inside the cell. Localization and interaction studies can be performed, and concentrations of ions and intra- and extracellular processes such as endocytosis and exocytosis can be observed.

Also, the fluorescence microscope is applicable for

  • Marking of molecules in cells and tissues for characterization and identification.
  • Normal and pathological cell study.
  • Immunology studies.
  • Mineralogy.

Kalstein brand microscope

At Kalstein, we are equipped with the most extensive technology in trained laboratory equipment, to meet all the demands of our customers. In this case, we offer you the Microscopes, belonging to the YR models, with general and very attractive features, such as; Infinite Optical System, Infinity Siedentopf Binocular Display Head; inclined to 45ยฐ interpupillary 47-78 mm, Infinite Phase Contrast Lens, NA1.25 Abbe Inserted Condenser (including empty plate), 3 W S-LED (LCD screen amplification), suspension timer, brightness lock and indication, etc., Two wave range (B, G, U, V can be combined) Fly-eye lens illumination. application software. Tilt binocular display head. Fivefold backward (no coding). Scenario without frame 230 mm x 150 mm, range of motion 78 mm x 54 mm

We are manufacturers and we have the best advice, so that your purchase is the ideal and at excellent prices. For more information, visit our website at https://kalstein.co.in/



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