Technological advances have driven revolutionary changes in several disciplines, including molecular biology, characterized by the intensive use of tools such as electrophoresis. This technique, along with its intrinsic ally, gel documentation, has completely transformed the landscape of genetic research, but its optimal use still presents a challenge in many laboratories.
The electrophoresis gel documentation system is an essential tool that allows the comparison and analysis of separated DNA, RNA and proteins by electrophoresis on agarose or polyacrylamide gels. Its main purpose is to effectively record and store the results of electrophoretic runs. Its main components are: a transilluminator to visualize the gel, a camera to capture the image and software to process and store the image. However, the correct handling of this system is essential for the final validity and interpretability of the results.
Correctly Using the Electrophoresis Gel Documentation System
To maximize the effectiveness of the gel documentation system, several key guidelines must be followed. First, the gel must be prepared correctly, the pore size of the gel must be selected according to the size of the molecules to be analyzed. Then, it must be loaded without interruptions to avoid distortion of the final image. When viewing the gel, it is important to adjust the illumination to highlight the bands of interest without overexposing the image. Finally, when capturing the image, be sure to use the appropriate resolution to allow for detailed analysis later.
Interpreting gel electrophoresis results can be challenging, but with the gel documentation system, multiple samples can be analyzed and compared simultaneously. Banding patterns can be compared to a molecular weight marker to determine the size of the molecule. In addition, specific software can be used to quantify the amount of DNA or protein present in each band. This information can be essential for both basic research and clinical applications, such as the diagnosis of genetic diseases.
What Kalstein has for you
Electrophoresis gel documentation systems are valuable and powerful tools in biological research. Their utility is undeniable, allowing researchers to separate, visualize and interpret protein and nucleic acid samples with unprecedented accuracy and efficiency. However, proper handling and execution is required to maximize this utility. By following proper guidelines for image preparation, visualization, capture and interpretation, users can unlock the full potential of their electrophoresis gel documentation system and greatly improve the calibration and efficiency of their laboratory work.
We at Kalstein as a manufacturing company, also have for you a 3D platform, where users, manufacturers and distributors can sell, rent or offer new or used equipment anywhere in the world, enjoy being seen, be more. If you want to buy at the best price, visit us HERE where you will find the different Kalstein YR models of gel documentation systems, which are available for any requirement of health centers, in addition to having the best advisors to accompany you during the process.