The importance of the microplate washer is that during the ELISA a microplate washer is responsible for removing unbound material and reagents that have not reacted. Washing is a critical stage in the performance of the technique.
It is extremely essential not only to respect the number of wash cycles and buffer volumes in each well. The soaking time is standardized to reduce the interference produced by the samples, as well as to eliminate the conjugate not specifically bound to the reaction, and its objective is to provide sufficient time to allow the diffusion of the components to be eliminated in the buffer, thus making the washing performance more optimal. The washing processes carried out in the ELISA microplate washer include the following: top-down suction, simultaneous suction and dispensing and suction from the bottom of the wells.