The microplate reader is a laboratory kit developed for the quantification of proteins and nucleic acids through cell imaging. It relies on filtration of proteins deposited in wells of an adjusted plastic plate to assist absorption and bind antibodies.
In this case, the microplate reader is one of the methods applied to diagnose cancer, which starts with the blood sample and is based on the determined interaction between antigens and antibodies, where the discovery is executed through substances and enzymatic reactions.
And Kalstein has the ideal equipment, because thanks to its functions, it performs studies using the Elisa technique, where the microplate reader uses antibodies and measures the amount of the substance in serum where an enzymatic reaction occurs that changes color and records the results. This equipment is especially used to detect viral diseases. Fluorescence measurements detect absorbance, luminescence in a specialized way as time-resolved fluorescence (TRF) and fluorescence polarization (PF).
Microplate Reader Efficiency
Currently, it has been shown that in the majority of the female population the worldwide incidence of ovarian cancer is the most common cause of mortality after breast, colorectal, lung and uterine cancer, due to many factors such as age, menopause, diet, overweight, physical activity, continuing pregnancies, drugs, smoking, alcohol and family history. And thanks to the early diagnosis by the Elisa method applied by the microplate reader, provides the necessary and timely information for monitoring the disease.
Its functions are based on separating the immune complex created on the solid base and the unfixed particles, which is performed by aspiration and washing, facilitating the elimination of residues that may interfere with readings. Separation is done by sedimentation where the immune complexes of interest are taken, filtration, radial diffusion and other immunochromatographic principles that work for fast and simple practices.
In detail, the microplate reader offered by Kalstein allows the readings of the results to be reliable thanks to the external jet printer, shows the reports of the multiformat studies, because it also has the appropriate technology and an 8-channel optical system, which allows testing in a quantitative and qualitative way, and are the ideal methods to apply the different methods, both direct, indirect, secondary and sandwich type being the most recommended method for the automation of the Elisa technique.
Assessment of tumor markers. Elisa technique
Using the microplate reader and the Elisa technique, it will allow tumor markers to be determined through the following steps of extraction and analysis, which are:
- The sample should be removed venously and placed in a tube free of additives and coagulants.
- The blood has to clot.
- Centrifuge the samples at 1200 rpm for 10 mn.
- Using a pipette, transfer the centrifuged serum into prepared or encoded tubes.
- Separate serum samples into a bleb.
- Store samples in refrigerators at room temperature.
- Have the solutions for washing ready before use, diluting distilled water with amounts of 1000 mL, then calibrate and obtain the testing curve.
- Place samples in the microplate wells.
- Incubate for 1 hour at room temperature.
- Remove reaction mixture
- Incubate for 15 min at room temperature protected from light.
- Place sample into microplate wells
- Shake the microplate very carefully
- Finally, read the absorbance at 450 nm versus target within 5 mn.
In this way, the frequency of risk factors and tumor marker are obtained.
Kalstein Brand Microplate Reader
At Kalstein, we are manufacturers and pioneers in technological applications designed for our Microplate Readers, which we offer to our customers. They belong to the YR series, has a reading range of 0.000-4.000 Abs. 405nm, 492nm, 630nm wavelength. They provide connectivity and intelligence. HERE